Use of Eggshell Membrane as an Immobilization Platform in Microbial Sensing

Abstract

A new microbial biosensor was developed by immobilizing Pseudomonas fluorescens cells on eggshell membrane via physical adsorbtion. Bacteria-modified eggshell membrane was fixed tightly onto the surface of a carbon paste electrode (CPE) with a silicone rubber o-ring to construct a microbial biosensor. The measurements were based on the respiratory activity of the cells caused by oxygen consumption during bacterial metabolism. A mediated biosensor was also developed by ferrocene. As well as the response characteristics, stabilities and substrate specificities were investigated. Data were given as the comparison of mediated and unmediated systems.

Keywords:

• Eggshell membrane
• mediator
• microbial sensor
• pseudomonas fluorescens

Notes

Osmium redox polymer I: poly(1-vinylimidazole)₁₂-[osmium(4,4-dimethyl-2,2-bipyridyl)₂Cl₂]^2+/+, Osmium redox polymer II: poly(vinylpyridine)-[osmium-(N,N-methylated-2,2-biimidalzole)₃]^2+/3+, CNTEC: carbon-nanotube epoxy composite, HCF: potassium hexacyanoferrate (III).

∗x and y were in µM and µA.