Direct Determination of Uric Acid Based on Pd Nanoparticles Electrodepositing onto Anatase-Type TiO₂ Nanoparticles/Chitsan Film-Modified Electrode

Abstract

A solvothermal method has been used to synthesize anatase-type TiO₂ nanoparticles (NPs) using ionic liquid (1-ethyl-3-methylimidazolium-ethyl sulfate) as cosolvent and modifier. Palladium nanoparticles were electrodeposited on the glassy carbon (GC) electrode modified with synthesized TiO₂ nanoparticles/chitosan film in the presence of glycin. The resulting amperometric sensor was used for direct determination of uric acid in human serum samples. Ionic liquid, TiO₂, and Pd nanoparticles were characterized by infrared spectra (IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). This sensor offered an excellent detection for uric acid at 0.60 V in a linear response range of 4.42 × 10⁻⁵ to 7.98 × 10^–3 M with a correlation coefficient of 0.997. The sensor displays rapid response, expanded linear response range, excellent repeatability, and excellent stability.

Keywords:

• Ionic liquids
• TiO₂ nanoparticles
• electrodeposition
• Pd nanoparticles
• uric acid
• amperometric sensor

This work was supported by the Foundation of Science Commission of Yunnan Province (No. 2006B0028M) and the Foundation of Department of Education of Yunnan Province (No. 07Z10087).