Abstract
The mediated electrochemical method and the intrinsic NADH fluorescence method were employed in evaluating the catabolic activities of three yeasts. The responses from the menadione/ferricyanide system were 70.00 ± 2.25 nA, 61.39 ± 1.76 nA, and 57.18 ± 1.51 nA, respectively, for Saccharomyces cerevisiae, Pachysolen tannophilus, and Pichia stipitis. The NADH fluorescence intensities were 1638 ± 25.46 FI, 1039 ± 18.67 FI, and 963.4 ± 15.78 FI, respectively,for S. cerevisiae, P. tannophilus, and P. stipitis. It was evident that there is a positive relationship between the mediated electrochemical method and the intrinsic NADH fluorescence method in cellular metabolic activity assays.
Financial support by the National Natural Science Foundation of China (20675036) is gratefully acknowledged.