Application of Liquid Chromatography/LTQ Linear Ion Trap Mass Spectrometry for Quantifying the Biomarkers Creatinine and Cortisol in Serum

Abstract

Random metabolites present in a complex matrix can be characterized and quantified by a fast and robust liquid chromatography/mass spectra (LC/MS) method using the LTQ linear ion trap MS system. The experimental results showed excellent selectivity, precision, and sensitivity. The LTQ linear ion trap MS was found to be an excellent and highly selective instrument for measuring metabolites such as creatinine and cortisol present in complex matrices, even when such analytes were present at trace levels.

Keywords:

• Cortisol
• creatinine
• LC/MS
• LTQ
• quantification
• serum

This work was supported by NSFC20627004 and National Key Technologies Research and Development Program 2006BAF07B03. We thank Wu Liqing for the measurement of the mass spectra.

Notes

^a Standard deviation (SD) of a single measurement within a set.

^b SD of the mean for that level.

^a Standard deviation (SD) of a single measurement within a set.

^b SD of the mean for that level.