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Analytical Letters Volume 42, 2009 - Issue 1
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PHARMACEUTICAL ANALYSIS

Determination of Belotecan in the Plasma, Bile, and Urine of Rats by High-Performance Liquid Chromatography with Fluorescence Detection and Its Application to a Pharmacokinetic Study

Qing-Ri Jin National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea; Research Institute of Pharmaceutical Sciences and Department of Pharmaceutics, College of Pharmacy, Seoul National University, Seoul, Korea
,
In-Wha Kim National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea; Research Institute of Pharmaceutical Sciences and Department of Pharmaceutics, College of Pharmacy, Seoul National University, Seoul, Korea
,
Min-Koo Choi National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea; Research Institute of Pharmaceutical Sciences and Department of Pharmaceutics, College of Pharmacy, Seoul National University, Seoul, Korea
,
Won-Sik Shim National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea; Research Institute of Pharmaceutical Sciences and Department of Pharmaceutics, College of Pharmacy, Seoul National University, Seoul, Korea
,
Guang-Yan Tian Department of Veterinary Parasitology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
,
Soohyun Lee National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea; Research Institute of Pharmaceutical Sciences and Department of Pharmaceutics, College of Pharmacy, Seoul National University, Seoul, Korea
,
Jonghwa Lee Korea Institute of Toxicology, Daejeon, Korea
,
Dae-Duk Kim National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea
,
Suk-Jae Chung National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea
&
Chang-Koo Shim National Research Laboratory for Transporters, Targeted Drug Design, College of Pharmacy, Seoul National University, Seoul, Korea; Research Institute of Pharmaceutical Sciences and Department of Pharmaceutics, College of Pharmacy, Seoul National University, Seoul, KoreaCorrespondence[email protected]
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Pages 68-83 | Received 19 Sep 2008, Accepted 07 Oct 2008, Published online: 21 Jan 2009
 
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Abstract

A simple and reliable high-performance liquid chromatographic (HPLC) method was developed for the determination of belotecan in the plasma, urine, and bile samples of rats. Belotecan was analyzed with HPLC using a C18 column with fluorescence detector. A mixture of acetonitrile–0.1 M potassium phosphate buffer at pH 2.4 (25:75, v/v) and 0.2% trifluoroacetic acid was used as the mobile phase. The lower limits of quantitation (LOQ) were 5 ng mL−1 for the plasma and 5 µg mL−1 for the urine and bile samples. The method has been readily applied for the routine pharmacokinetic study of belotecan in small laboratory animals.

This study was supported by a grant from the Korean Ministry of Science and Technology through the National Research Laboratory Program (ROA-2006-000-10290-0).

Notes

a SD, standard deviation.

a SD, standard deviation.

a SD, standard deviation.

a AUC: area under the plasma concentration time curve; CL: clearance; Vss: apparent volume of distribution at a steady state; CLb: biliary clearance; CLr: renal clearance.

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