Gene Expression Profile of Prostate Cancer Patients by Chemiluminescent Analysis

Abstract

The heterogeneous and multifactorial nature of prostate cancer that generates differential gene expression patterns in tumor cells leads us to investigate the molecular mRNA profiling of 14 genes through streptavidin-alkaline phosphatase–labeled RNA probes from tissue samples with prostate cancer and benign prostatic hyperplasia. Hybridizations were performed using cDNA amplification for each gene spotted onto positively charged nylon membranes and densitometry readings. The constitutive gene GAPDH was used to normalize the data. The methods developed in this study may be applicable to the prostate cancer diagnosis using AR, CEACAM-1, DD3 (also called PCA3), OPN-1, and PSMA significant differential expression.

Keywords:

• Nucleic acid hybridization
• nucleotide probe
• reverse transcription-polymerase chain reaction

We thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Financiadora de Estudos e Projetos (FINEP), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), and Universidade Federal de Uberlândia (UFU) for funding support.

Notes

Note. SV = splicing variant.

^a,b Primer sequences described by Neves et al. (2008) and Gan et al. (2000), respectively, and the 378-bp product for KLK2 gene represents an alternative splicing described by Riegman et al. (1991).

^c Primer sequence described by Hibi et al. (1998).