Enoxacin—Tb³⁺ Complex as an Environmentally Friendly Fluorescence Probe for Coenzyme A and Its Application

Abstract

A new spectrofluorimetric method was developed for the determination of trace amounts of coenzyme A using enoxacin–Tb³⁺ as a fluorescent probe. In the presence of periodic acid (H₅IO₆), coenzyme A could remarkably enhance the fluorescence intensity of the Tb³⁺–enoxacin complex at 545 nm at pH 5.4. The optimal conditions for the determination of coenzyme A were also investigated. This method could be successfully applied to assess coenzyme A in injection and biological samples. Moreover, the enhancement mechanism of the fluorescence intensity of the coenzyme A–Tb³⁺–enoxacin system in the presence of H₅IO₆ was also discussed.

Keywords:

• Enoxacin
• luminescence mechanism
• spectrofluorimetry
• terbium

The authors acknowledge the support from the National Natural Science Foundation of China (20805016), the Science Foundation of Huazhong University of Science and Technology (0101013008), and the Science Foundation of Huaibei Coal Industry Teachers' College.

Notes

^a 1, Changzhou Qianhong Biochemical Pharmaceutical Co., Ltd., Changzhou, China (no. H32022090); 2, Jiangsu Wuzhong Industry Stock Co., Ltd., Jiangsu, China (no. H32022800); and 3, Hengyang Hengsheng Pharmaceutical Co., Ltd., Hengyang, China (no. H43020632).

^b The weight of the contents in an ampoule.

^c Relative standard deviation (n = 5).