Simultaneous Separation and Determination of Different Polar Flavonoids in Multiflora Fruit by Reverse-Phase High-Performance Liquid Chromatography

Abstract

A method for the determination of different polar flavonoids from multiflora fruit by reverse-phase high-performance liquid chromatography (HPLC) is presented in this article. Chromatographic separation of these flavonoids was performed over a Diamonsil C₁₈ column (200 mm × 4.6 mm × 5 µm) using gradient elution with an isopropanol–methanol–water mixture at 25°C, at a flow rate ranging from 0.5 to 1.5 mL/min and detection at 360 nm. The flavonoids could be separated within 6 min; the recovery was between 96% and 104%, and the relative standard deviation was 1.6–2.6%. This method allows rapid detection of flavonoids.

Keywords:

• Flavonoids
• fruit analysis
• gradient elution
• mobile phase composition
• reverse-phase HPLC

Acknowledgments

The authors gratefully acknowledge the support from the National Nature Science Foundation of China (20675010), the Specialized Research Fund for the Doctoral Program of Higher Education 200800270006 and the Beijing Natural Science Foundation (2093036).

Notes

^a total amount of Rosa multiflora;

^b the amount of each flavonoids per gram Rosa multiflora