Interference of the Simultaneous Presence of Different Biogenic Amines on the Response of an Amine Oxidase–Based Biosensor

Abstract

The application of an amine oxidase–based biosensor, which was selective for several biogenic amines, was examined for the simultaneous detection of cadaverine, putrescine, and histamine. The biosensor response parameters in mixtures containing cadaverine or putrescine were 1.14 times less than the sum of corresponding parameters in single-substrate solutions. Histamine did not cause any change of the biosensor response in the presence of cadaverine and/or putrescine. This “screening” of biosensor signal parameters by putrescine and cadaverine should be considered during calibration of amine oxidase–based biosensors used for the detection of biogenic amines.

Keywords:

• amine oxidase
• biogenic amines
• biosensor response
• oxygen sensor
• screening

Acknowledgments

This work was supported by the Estonian Science Foundation (Grant No. 7465). Kairi Kivirand was supported by a World Federation of Scientists grant.