Preliminary Recognition of c-Myc Gene Protein Using an Optical Biosensor with Gold Colloid Nanoparticles Based on Localized Surface Plasmon Resonance

Abstract

The gold colloidal nanoparticles with a diameter of 24 ± 0.2 nm were prepared, characterized by ultraviolet–visible (UV-vis), transmission electron microscopy (TEM), and cyclic voltammetry, and assembled on a gold plate substrate for constructing an optical nanobiosensor. The nanosensor exhibited distinct optical properties of localized surface plasmon resonance (LSPR), which could be used for recognition of oncogene biomolecules such as the c-Myc (3C7) antibody protein. The LSPR nanobiosensor has also been successfully applied to determination of a pCMV-Myc mammalian expression vector, one kind of DNA plasmids with a linear response range of 6.2–20.0 ng/µL and a detection limit of 2.4 ng/µL.

Keywords:

• Gold nanoparticles
• localized surface plasmon resonance
• oncogene protein
• optical nanobiosensor
• pCMV-Myc

This work has been supported by the projects of the National Natural Science Foundation of China (Nos. 20775010, 20873148, 50671098, and U0734005), the Hunan Provincial Natural Science Foundation of China (Nos. 09JJ3016 and 09JJ3035), the Scientific Research Fund of Hunan Provincial Education Department of China (Nos. 08B004, 07A006), the Key Project of Chinese Ministry of Education (No. 208095), 863 projects of China (Nos. 2008AA100801 and 2007AA05Z102), and the Hunan Provincial Innovation Foundation for Postgraduate Study, China.