Abstract
This article describes the development and validation of a first derivative UV quantitative analytical method for determination of candesartan cilexetil in tablet dosage forms. A signal at 270.1 nm of the first derivative spectrum (ID270.1) was found adequate for quantification. The limit of quantification was 3.06 µg/ml. The linearity between ID270.1 nm and concentration of candesartan cilexetil in the range of 6.00–32.00 µg/ml presented a correlation coefficient of (r2) = 0.9990. The mean recovery percentage was 100.97 and 99.23% for candesartan cilexetil standard solution and candesartan standard cilexetil solution with excipients, respectively. The intraday and interday accuracy of the assay was 98.60% and 99.10% respectively. The intraday and interday variability was below 2.0%.
The proposed method is accurate, precise, sensitive, and selective and can be used in quality control laboratories for its intended purpose.
Notes
The Standard error of the slope is 9.46e–06.
The Standard error of the intercept is 0.0002052.
The square correlation (r2) = 0.9990.
The regression standard deviation Sy\x RMSE = 0.00021.
Linear regression equation is; ID270.1 = −0.0006862 concentration + (−0.0008371).
LOD = 0.92 µg/ml.
LOQ = 3.06 µg/ml.