Determination of Candesartan Cilexetil in Tablet Dosage Forms and Dissolution Testing Samples by First Derivative UV Spectrophotometric Method

Abstract

This article describes the development and validation of a first derivative UV quantitative analytical method for determination of candesartan cilexetil in tablet dosage forms. A signal at 270.1 nm of the first derivative spectrum (ID_270.1) was found adequate for quantification. The limit of quantification was 3.06 µg/ml. The linearity between ID_270.1 nm and concentration of candesartan cilexetil in the range of 6.00–32.00 µg/ml presented a correlation coefficient of (r²) = 0.9990. The mean recovery percentage was 100.97 and 99.23% for candesartan cilexetil standard solution and candesartan standard cilexetil solution with excipients, respectively. The intraday and interday accuracy of the assay was 98.60% and 99.10% respectively. The intraday and interday variability was below 2.0%.

The proposed method is accurate, precise, sensitive, and selective and can be used in quality control laboratories for its intended purpose.

Keywords:

• Candesartan cilexetil
• first derivative spectrophotometry
• tablet dosage form

Notes

The Standard error of the slope is 9.46e–06.

The Standard error of the intercept is 0.0002052.

The square correlation (r²) = 0.9990.

The regression standard deviation S_y\x RMSE = 0.00021.

Linear regression equation is; ID_270.1 = −0.0006862 concentration + (−0.0008371).

LOD = 0.92 µg/ml.

LOQ = 3.06 µg/ml.