A 96-well Plate Based Dot-ELISA Array for Simultaneous Detection of Multi-Drugs

Abstract

Drug abuse is rising day by day and becoming a big problem in countries around the globe. The existing drug testing methods could not effectively cope with the problems in the sensitivity and specificity of the diversified drugs. Dot-ELISA, as a complement of ELISA, has been widely used in many fields. In this study, we optimized and applied the Dot-ELISA technique as a maneuverable assay for rapid detection of multiple drug abuses via a kind of 96-well plates-based Dot-ELISA array. In total 92 positive urine samples were tested with high-throughput and all results were confirmed by commercial drug testing kits.

Keywords:

• Dot-ELISA array
• high-throughput
• multiple detections of drug abuse

This study has been supported by grants from the Beijing Nova Programme of Science and Technology (No. 2007B024) and the Original Foundation of the Ministry of Security of the People's Republic of China (No. 2006YYCXBJSJ123).