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Analytical Letters Volume 43, 2010 - Issue 2
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BIOANALYTICAL

Determination of Listeria Monocytogenes in Milk Samples by Signal Amplification Quartz Crystal Microbalance Sensor

Huanying Zhou Institute of Hygienic and Environmental Medicine, Tianjin, China; Tianjin Key Laboratory of Risk Assessment and Control for Environment & Food Safety
,
Zhixian Gao Institute of Hygienic and Environmental Medicine, Tianjin, China; Tianjin Key Laboratory of Risk Assessment and Control for Environment & Food SafetyCorrespondence[email protected]
,
Guihua Luo Institute of Hygienic and Environmental Medicine, Tianjin, China; Tianjin Key Laboratory of Risk Assessment and Control for Environment & Food Safety
,
Le Han Institute of Hygienic and Environmental Medicine, Tianjin, China; Tianjin Key Laboratory of Risk Assessment and Control for Environment & Food Safety
,
Siming Sun Institute of Hygienic and Environmental Medicine, Tianjin, China; Tianjin Key Laboratory of Risk Assessment and Control for Environment & Food Safety
&
Hongyong Wang Institute of Hygienic and Environmental Medicine, Tianjin, China; Tianjin Key Laboratory of Risk Assessment and Control for Environment & Food Safety
Pages 312-322 | Received 13 Jan 2009, Accepted 08 Aug 2009, Published online: 13 Jan 2010
 
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Abstract

A piezoelectric crystal biosensor method has been established for detection of listeria monocytogenes (LM) by gold nanoparticles (GNPs) signal amplification. Based on specific recognition of single-stranded DNA (ssDNA) hybridization, a sandwich format of ssDNA probes/complimentary ssDNA probes modified by GNPs/ssDNA of LM was fabricated. This novel strategy allows the detection limit of LM as low as 10 cfu/mL. The biosensor showed friendly biocompatibility, good specificity, acceptable stability, which provided a potential alternative tool for detection of LM in real samples. Furthermore, the developed method could be easily extended to other gene analysis schemes.

The authors gratefully acknowledge the financial support by Natural Science Foundation of China (No. 30571566) and the Natural Science Foundation of Tianjin (No. 053607111, 09JCYBJC08300).

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