Human p53 and Hdm2: Cloning and Construction of Expression Plasmid

Abstract

The research work reported herein is the cloning and the construction of the expression plasmid of human p53 and Hdm2. The cloning of p53-cDNA and Hdm2-cDNA based on RT-PCR reactions allows for the construction of expression plasmid using the pET-28a (+) transfer vector for the purpose of obtaining recombinant human p53 and Hdm2 in bacteria. The obtained recombinant p53 and Hdm2 proteins will be useful for identifying potent low molecular-weight compounds that block the p53/Hdm2 interaction and permit the activation of p53 and, as a consequence, show anticancer activity.

Keywords:

• Cloning
• Expression plasmid
• Hdm2
• p53