High Performance Liquid Chromatography—Tandem Mass Spectrometric Determination of Resveratrol and Its Metabolites in Rat Tissues

Abstract

In this study, we established a rapid, sensitive, and specific liquid chromatography method coupled with electrospray ionization–tandem mass spectrometry to evaluate the disposition of resveratrol and its metabolites in rat tissues following oral administration. Here, we validate this method and show that it is useful for quantifying tissue concentrations of resveratrol and its metabolites with acceptable specificity, linearity, lower limits of quantification, precision, accuracy, and recovery. The present study demonstrates that the major distribution of the trans-resveratrol and its metabolites in investigated tissues, which suggests that there is a specified range of target organs for cancer chemoprevention by polyphenols.

Keywords:

• Disposition
• LC/ESI-MS/MS
• Metabolism
• Polyphenols
• Resveratrol

Acknowledgments

This work was supported, in part, by grants from the National High-Tech Research and Development Program of China (863 Program), No. 2003AA2Z347C.

Notes

X. Liu and Z. Teng and contributed equally to this work