The Surfactin and Lichenysin Isoforms Produced by Bacillus licheniformis HSN 221

Abstract

Surfactin and lichenysin are typical members of the lipopeptide family. Four surfactin and two lichenysin isoforms were isolated by Pre RP-HPLC from the cell-free broth of Bacillus licheniformis HSN 221 cultivated in YPD medium. The molecular weight of each isoform was determined by ESI-MS. The peptide part of each isoform was elucidated by Q-Tof MS/MS combined with HPLC, and the fatty acid part was analyzed by GC/MS. It showed that the four surfactin isoforms have the primary structures of anteiso(iso)C₁₃, isoC₁₄, anteiso(iso)C₁₅, anteiso(iso)C₁₅-surfactin-methyl ester, and the two lichenysin isoforms have those of nC₁₄ and anteiso(iso)C₁₅, respectively.

Keywords:

• Bacillus licheniformis
• Isoforms
• Lichenysin
• Lipopeptide
• Surfactin

This work was supported by the National Natural Science Foundation of China (No. 50744016), the grant from the Ministry of Science and Technology of China (2007CB707801), and Shanghai Municipal Science and Technology Commission (071607014).

Notes

∗Represents weak peaks not labeled in the figure; FA represents fatty acid.

∗Represents weak peaks not labeled in the figure; FA represents fatty acid.