Peroxidase in Chemical and Biochemical Analysis

Abstract

The structure, reaction kinetics and mechanism, substrate specificity, stability and chromogenic, and fluorogenic and chemiluminogenic substrates of peroxidase are reviewed. The applications of peroxidase in the quantitative analysis of hydrogen peroxide, clinically important metabolites via oxidase-coupled reactions, as a label in quantitative immunochemical and DNA hybridization reactions and in immuno-blot and immuno-histochemical investigations will also be reviewed.

The author acknowledges Dr. Van T. Lieu, Professor emeritus of Analytical Chemistry, California State University at Long Beach, California, for critical review of this manuscript and thanks him for constructive comments.

This article was submitted as part of a Special Memorial Issue honoring Prof. George G. Guilbault.