A Novel Enhanced Chemiluminescence System with Ag(III) Complex for the Determination of Ofloxacin and Levofloxacin in Pharmaceutical Preparation and Biological Fluid

Abstract

A novel chemiluminescence (CL) method is developed for determination of ofloxacin and levofloxacin with Ag(III) complex in H₂SO₄ solution medium. The CL intensity is proportional to drug concentration in a wider range with a correlation coefficient of 0.999. The limit of detection (s/n = 3) for ofloxacin and levofloxacin was 5.3 × 10⁻⁹ g ml⁻¹ and 8.3 × 10⁻⁹ g ml⁻¹, respectively, and their recoveries from urine and serum samples were in the range of 90.1–112% with the RSDs of 1.0–2.8%. The proposed method was applied for analysis of real samples with satisfactory result. The possible CL mechanism was discussed.

Keywords:

• Ag(III) complex
• Biological fluid
• Chemiluminescence
• Flow-injection
• Levofloxacin
• Ofloxacin
• Pharmaceutical preparations

This work was supported by the Science Foundation Education Office of Hebei Province (B2008000583).

Notes

*n = 5.

*n = 5, **no detected.