Abstract
A new composite film comprising cationic gemini surfactant butane-α,ω-bis(dimethyl dodeculammonium bromide) (BDDA, C12-C4-C12) and poly (allylamine) hydrochloride(PAH) have been prepared. The composite film showed good biocompatibility and could promote the direct electron transfer between hemoglobin (Hb) and glassy carbon (GC) electrode. The immobilized Hb exhibited a pair of well-defined, quasi-reversible, and stable redox peaks with a formal potential of −0.158 V (vs. SCE) in 0.10 M pH 7 phosphate buffer solutions, and showed high affinity to hydrogen peroxide. The cathodic peak current of the electrode was linear with increasing concentration of H2O2 in the range of 5.14 to 200 μM.
Acknowledgments
This work was supported by Specialized Research Fund for the Doctoral Program of Higher Education (No. 20060532006) and National Outstanding Youth Foundation of China (No. 50725825).