Determination of Methadone in Human Hair by Headspace Extraction and Ion Mobility Spectrometry

Abstract

Ion mobility spectrometry along with headspace extraction was used for the determination of methadone in human hair. The hair samples were digested in an extraction cell containing NaOH and NaCl. Then, headspace extraction and determination processes were continuously performed. The calibration curve was linear over a range of 0.20 to 25 ng mg⁻¹ with a correlation coefficient of 0.987. The limit of detection and the limit of quantitation were 0.01 and 0.03 ng mg⁻¹, respectively. The relative standard deviation and the extraction recovery were 8.7% and about 70%, respectively. The developed method was applied to the analysis of real samples.

Keywords:

• Headspace extraction
• Human hair
• Ion mobility spectrometry
• Methadone

Acknowledgments

This work has been supported by Isfahan University of Technology of Iran.

Notes

^a After 10 min.

^a Linear dynamic range.