Determination of Vitamins A and E Exploiting Cloud Point Extraction and Micellar Liquid Chromatography

Abstract

Cloud point extraction and micellar chromatographic methods were developed for determination of vitamins A and E. The stationary phase was C18 and the mobile phase was 3.00% (w/v) SDS, 15.0% (v/v) butyl alcohol and 0.02 mol L⁻¹ phosphate buffer solution at pH 7.0. The retention times for vitamins A and E were 9.6 and 13.0, respectively. The extraction solution was 100 mmol L⁻¹ Triton X-100, 650 mg NaCl and 1.0% ascorbic acid at 70°C for 30 min. The method is precise (r.s.d. < 7%), the linear range was from 5.0 up to 360.0 mg L⁻¹ for both vitamins. Recovery test showed recuperation between 90.2 and 99.2%, and LOD and LOQ of 0.234 and 0.108 mg L⁻¹, 0.780 and 0.360 mg L⁻¹ to vitamins A and E were found.

Keywords:

• Cloud point extraction
• HPLC
• Vitamins A and E

Notes

Extraction conditions: time interval of 30 min, 650 mg of NaCl at 70°C.

The results are expressed in mg g⁻¹ and mg L⁻¹ ± the standard deviation (for oil and soft drink samples, respectively).