Spectrochemical Characterization of Thin Layers of Lipoprotein Self-Assembled Films on Solid Supports Under Oxidation Process

Abstract

Low density lipoprotein self-assembled layers on gold support, proposed as model for oxidation studies, were subjected to oxidation processes using different oxidative agents: 2,2′-Azobis(2methylpropionamidine)dihydrochloride, atmospheric oxygen, and metal-induced oxidation. The freshly prepared and the oxidized layers were characterized by X ray photoelectron spectroscopy (XPS), Fourier-Transformed infrared spectroscopy, and Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-ToF) mass spectrometry to discriminate the effects of oxidative reagents. Data obtained from FTIR and MALDI spectra proved the lipoperoxide formation subsequent to reactive oxygen species attack and the opportunity to use the model to discriminate between oxidation toxicity.

Keywords:

• AAPH
• FTIR
• Lipoprotein peroxidation
• MALDI-ToF
• XPS

Acknowledgments

Andreia Tache greatly acknowledges the kind support of the Department of Chemistry, Università degli Studi di Bari, Italy.

This work was financially supported by project IDEI, contract no 241/2007.