Advanced search
Publication Cover
Analytical Letters Volume 14, 1981 - Issue 17-18
Submit an article Journal homepage
13
Views
5
CrossRef citations to date
0
Altmetric
Original Articles

α-Amylase Purification and Separation from Gluco-Amylase by Affinity Chromatography on Cross-Linked Amylose(CL-Amylose)

Horst D. Schill Institute of Biological Sciences, Laboratory of Molecular Biology, Splaiul Independentei 296 77748, Bucharest, 17, ROMANIA
,
Mircea A. Mateescu Institute of Biological Sciences, Laboratory of Molecular Biology, Splaiul Independentei 296 77748, Bucharest, 17, ROMANIA
,
Traian Bentia Institute of Biological Sciences, Laboratory of Molecular Biology, Splaiul Independentei 296 77748, Bucharest, 17, ROMANIA
&
Aurora Jifcu Institute of Biological Sciences, Laboratory of Molecular Biology, Splaiul Independentei 296 77748, Bucharest, 17, ROMANIA
Pages 1501-1514 | Received 30 Jun 1981, Accepted 09 Sep 1981, Published online: 24 Feb 2007
 
Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days

Abstract

Using a CL-amylose column it is possible to purify α-amylase from other protein contaminants (e.g. albumin, haemoglobin, etc.) and from interfering exoamylases, by its specific retention on CL-amylose. α-Amylase is eluted off the column in the second step by a mixture containing 0.1 M NaCl in acetic acid 0.01 M and CaCl2 0.015 M. The recoveries of glucoamylase and α-amylase were 74% and 65% respectively. The retention capacity of the stationary phase is 0.5 mg of α-amylase/ml bed volume.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related Research Data

The use of cross-linked amylose in establishing the α- or β-amylase activity of an amylolytic preparation
Source: Elsevier BV
On amylolytic enzymes; beta-amylase; activity assay and control of the absence of alpha-amylase.
Source: Wiley
Utilisation de l'amylose réticulée pour la détermination quantitative de l'α- et de la β-amylase en présence dans une préparation amylolytique
Source: Elsevier BV
Isolation of α-amylase from human and hog pancreas and saliva by affinity chromatography on a modified substrate
Source: Springer Science and Business Media LLC
Simultaneous Determination of Associated α- and Glucoamiuse Using Cross-Linked Amylose
Source: Informa UK Limited
Competitive affinity chromatography of wheat α-amylase
Source: Published by Elsevier B.V.
6 Plant and Animal Amylases
Source: Elsevier
Purification d'α-amylases par chromatographie d'affinité sur amidon réticulé
Source: Elsevier BV

Linking provided by 

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.