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ELECTROCHEMISTRY

A Biosensor for ADP with Internal Substrate Amplification

, , , &
Pages 657-668 | Received 27 Jan 1987, Accepted 12 Feb 1987, Published online: 06 Dec 2006
 

Abstract

An enzyme electrode was constructed from an oxygen electrode and a layer incorporating four enzyme systems for the sensitive determination of ADP and ATP. The cofactor is cycled between pyruvate kinase and hexokinase under formation of pyruvate which is detected by the coimmobilized, sequentially acting enzymes lactate dehydrogenase and lactate monooxygenase. The enzymatic recycling results in a 220-fold increased sensitivity to ADP compared to the unamplified reaction.

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