Simultaneous Identification and Determination of Several Barbiturates in Plasma by Reverse-Phase HPLC

M. López-rivadulla Department of Legal Medicine, Forensic Toxicology Service. Faculty of Medicine, C/ San Francisco, S/N., Santiago de Compostela, Spain

P. Fernández Department of Legal Medicine, Forensic Toxicology Service. Faculty of Medicine, C/ San Francisco, S/N., Santiago de Compostela, Spain

P. Rodríguez Department of Legal Medicine, Forensic Toxicology Service. Faculty of Medicine, C/ San Francisco, S/N., Santiago de Compostela, Spain

A. M. Bermejo Department of Legal Medicine, Forensic Toxicology Service. Faculty of Medicine, C/ San Francisco, S/N., Santiago de Compostela, Spain

L. Concheiro Department of Legal Medicine, Forensic Toxicology Service. Faculty of Medicine, C/ San Francisco, S/N., Santiago de Compostela, Spain

Abstract

A Reverse-Phase HPLC method is described for the simultaneous identification and determination of 5 barbiturates: Allobarbital, Phencbarbital, Butabarbi -tal, Butalbital and Pentobarbital. Barbital is used as internal standard. The mobile phase is Milli Q Water/ Acetonitrile/ 1.75 M Phosphoric Acid (738:200:2, v/v/v) at a flow rate of 0.8 mL/min and UV detection is carried out at 195 nm. The single extraction procedure requires only small samples and analysis is quick.

Key Words:

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Simultaneous Identification and Determination of Several Barbiturates in Plasma by Reverse-Phase HPLC

Information for

Open access

Opportunities

Help and information

Simultaneous Identification and Determination of Several Barbiturates in Plasma by Reverse-Phase HPLC

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature