Amperometric Determination of Catalase in Brazilian Commercial Honeys

Abstract

A sensor for catalase (CAT) amperometric detection in association with flow injection analysis and a tubular reactor containing Amberlite IRA-743 resin was developed. Catalase was quantified in 14 samples of Brazilian commercial honeys. At flow rate of 1.5 mL min⁻¹ and injecting 200 µL of sample volumes, a sampling frequency of 90 determinations per hour was afforded. The linear dynamic range in CAT extends from 50 to 5000 UI mL⁻¹, at pH 7.4. The levels of CAT vary from 9.97 to 99.07 UI mg⁻¹. Taking into account these results, an inverse correlation was obtained with CAT and H₂O₂ levels.

Keywords:

• Amperometry
• Catalase
• Honey

Acknowledgments

This paper was submitted as part of a Special Issue on Flow Injection Analysis.

Authors would like to thank FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), CAPES and PROPESQ/UFJF (Pró-Reitoria de Pesquisa e Pós-Graduação da Universidade Federal de Juiz de Fora) for financial support and grants.