Determination of Trenbolone Acetate in Cattle Feeds by a Flow Injection Chemiluminescence Method

Abstract

A flow injection chemiluminescence (CL) method is described for the determination of trenbolone acetate based on the CL generated during its reaction with KMnO₄ in acidic medium. The CL intensity is greatly enhanced by alizarin yellow R. The CL intensity is linear with trenbolone acetate concentration in the range 0.1–100.0 mg L⁻¹ with a detection limit of 0.05 mg L⁻¹. The sample throughout is about 90 h⁻¹ and the relative standard deviation for 2.0 mg L⁻¹ trenbolone acetate solution is 1.5% (n = 11). The proposed method was applied to the determination of trenbolone acetate in cattle feeds.

Keywords:

• Cattle feed
• Chemiluminescence
• Flow injection
• Trenbolone acetate

Acknowledgments

This paper was submitted as part of a Special Issue on Flow Injection Analysis.

The authors gratefully acknowledge financial support from Xi'an Jiaotong University (Grant No. 08140009).

Notes

^a I is the CL signal in presence of 2.5 mg L⁻¹ trenbolone acetate and I _b is the blank signal, ΔI = I − I _b .

^b n = 3.