Flow Injection Chemiluminescence Determination of Prednisone Acetate by Oxidation with N-Bromosuccinimide

Abstract

A simple flow injection chemiluminescence (CL) method for the determination of prednisone acetate was developed. This method is based on the fact that the strong CL of N-bromosuccinimide (NBS) and calcein can be inhibited by prednisone acetate. The CL intensity correlated linearly with the concentration of prednisone acetate over the ranges of 0.02–0.4 mg/L and 0.4–10 mg/L. The CL mechanism was also proposed. The detection limit (3σ) of prednisone acetate was 13 ng/mL and the relative standard deviation was 1.3% at 0.1 mg/L prednisone acetate (n = 11 measurements). This method was applied to the determination of prednisone acetate in the tablets with good results.

Keywords:

• Calcein
• Chemiluminescence
• N-bromosuccinimide
• Prednisone acetate

Acknowledgments

This paper was submitted as part of a Special Issue on Flow Injecion Analysis.

The authors are grateful for the financial support of the Natural Science Foundation of China (No. 20575035) and the University of Jinan (No. XBS0908).

Notes

^a Average of five determinations.