Sequential Injection Chemiluminescence Methodology for Ozone Evaluation

Abstract

A sequential injection methodology with chemiluminescence detection for the evaluation of residual ozone in waters is presented. The procedure is based on the reaction between luminol and ozone without catalysts.

Linear calibration plots were obtained for ozone concentrations between 0.05 and 2.0 mg L⁻¹, with a detection limit of 0.04 mg L⁻¹.

The developed methodology was applied to the determination of residual ozone in ozonized waters and the results complied with those furnished by the spectrophotometric reference procedure (relative deviations < 6.3%). The method exhibited good precision (RSD < 3.5%) and the sampling rate was about 140 determinations per hour.

Keywords:

• Chemiluminescence
• Luminol
• Ozone
• Ozonized water
• Sequential injection

Acknowledgments

This paper was submitted as part of a Special Issue on Flow Injection Analysis.

The authors wish to thank to GRICES (Portugal) and CAPES (Brazil) for the financial support for the exchange of researchers and the PhD grant. The authors are grateful to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and NQA (PRONEX, FAPESB–CNPq) for providing grants and fellowships and for the financial support.

Notes

^a SD – standard deviation of four replicates.

^b SD – standard deviation of three replicates.