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Analytical Letters Volume 44, 2011 - Issue 6
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MASS SPECTROMETRY

Application of Chip-Based Nanoelectrospray Ion Trap Mass Spectrometry to Compositional and Structural Analysis of Gangliosides in Human Fetal Cerebellum

Cristina Mosoarca Mass Spectrometry Laboratory, National Institute for Research and Development in Electrochemistry and Condensed Matter, Timisoara, Romania; Department of Biochemistry, “Victor Babeş” University of Medicine and Pharmacy Timişoara, Timişoara, Romania
,
Roxana M. Ghiulai Mass Spectrometry Laboratory, National Institute for Research and Development in Electrochemistry and Condensed Matter, Timisoara, Romania; Department of Biochemistry, “Victor Babeş” University of Medicine and Pharmacy Timişoara, Timişoara, Romania
,
Cristina R. Novaconi Mass Spectrometry Laboratory, National Institute for Research and Development in Electrochemistry and Condensed Matter, Timisoara, Romania
,
Željka Vukelić Department of Chemistry and Biochemistry, University of Zagreb Medical School, Zagreb, Croatia
,
Adrian Chiriac Department of Biochemistry, “Victor Babeş” University of Medicine and Pharmacy Timişoara, Timişoara, Romania
&
Alina D. Zamfir Mass Spectrometry Laboratory, National Institute for Research and Development in Electrochemistry and Condensed Matter, Timisoara, Romania; Department of Chemistry and Biology, ‘Aurel Vlaicu’ University of Arad, Arad, RomaniaCorrespondence[email protected]
Pages 1036-1049 | Received 11 Feb 2010, Accepted 12 Mar 2010, Published online: 31 Mar 2011
 
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Abstract

Two native ganglioside mixtures from normal human fetal cerebellum in the 15th (Cc15) and 40th (Cc40) gestational week were subjected to NanoMate high capacity ion trap (HCT) mass spectrometric (MS) and collision induced dissociation (CID) tandem MS (MS2) analysis under thoroughly optimized experimental conditionns. An total of 56 different species were identified in Cc15 and 54 in Cc40. By employing CID MS2 molecular ions, related GD1 (d18:1/20:0) and GM2 (d18:1/19:0) species were structurally characterized in a high throughput mode. The method provided elevated ionization efficiency, high speed of analysis, almost 100% reproducibility at sample consumption per experiment situated in the femtomole range.

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Corrigendum

Acknowledgments

This work was supported by the Romanian National Authority for Scientific Research through the project PN-II-41001/2007 to A. D. Zamfir and by the Croatian Ministry of Science and Technology under project 108120 to Ž. Vukelić. Permission for experiments with human tissue for scientific purposes was obtained from the Ethical Commission of the Medical Faculty of University of Zagreb, Croatia.

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