Identification of the Metabolites of Angiotensin II (1-8) in MES-13 Cells by a Simple NanoLC-MS-MS System: A Preliminary Platform

Abstract

The renin-angiotensin system (RAS) participates in regulating the body fluid, such as angiotensin II (1-8), and plays an important role in blood pressure control. In RAS, angiotensin II (1-8) is the major effector but other angiotensins may also mediate the actions of the RAS. In this study, a simple method is proposed for analysis of the metabolites of angiotensin II (1-8). Experimental data was acquired by a nanoLC system connected with quadruple time-of-flight (Q-TOF) mass spectrometry. The flow rate of this method was at 400 nL/min, and this nanoliter level flow rate would lower the consumption of organic solvents.

Keywords:

• Angiotensin II (1-8)
• Metabolites
• Nanoliter level
• Renin-angiotensin system

Acknowledgments

The authors are grateful to the National Science Council (NSC 98-2113-M-037-010 and 97-2113-M-037-008-MY2), Kaohsiung Medical University (Q-097021 and Q-098004), and Center of Excellence for Environmental Medicine (Kaohsiung Medical University) for financial support of this work. The authors are also grateful to the Center for Resources, Research and Development (Kaohsiung Medical University) for instrumental support of this work.

Notes

^a Sequence ions are based on the rules of peptide fragmentation in MASCOT search engine (http://www.matrixscience.com).

^b Peptide sequence.