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Analytical Letters Volume 44, 2011 - Issue 7
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BIOSENSORS

Voltammetric Biosensor Based on Acetylcholinesterase and Different Immobilization Protocols: A Simple Tool for Toxic Organophosphate Assay

Miroslav Pohanka Centre of Advanced Studies and Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic; Department of Toxicology, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech RepublicCorrespondence[email protected]
,
Oto Drobik Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Charles University in Prague, Hradec Kralove, Czech Republic
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Zuzana Krenkova Centre of Advanced Studies and Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic; Department of Toxicology, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
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Jana Zdarova-Karasova Department of Toxicology, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
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Jiri Pikula Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic
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Jiri Cabal Department of Toxicology, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
&
Kamil Kuca Centre of Advanced Studies and Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic; Department of Toxicology, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
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Pages 1254-1264 | Received 31 Jan 2010, Accepted 20 Apr 2010, Published online: 14 Jun 2011
 
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Abstract

The present study is aimed at construction of biosensors consisting of a screen printed sensor with a platinum working electrode and immobilized acetylcholinesterase (AChE) as a biorecognition element. Several immobilization protocols such as precipitation with glutaraldehyde, immobilization onto spherical graphite microparticles, sorption, interception into gelatin, and sol-gel were used in order to construct biosensors suitable for practical performance. The activity of AChE was evaluated by means of voltammetric oxidation of thiocholine originating from acetylthiocholine. Square wave and cyclic voltammetry were used throughout the experiments. Suitability of the constructed biosensors was evaluated using the toxic organophosphate diisopropylfluorophosphate.

Acknowledgments

Ministry of Defence of the Czech Republic is gratefully acknowledged for projects No. OVUOFVZ200807 and OVUOFVZ200801.

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