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Analytical Letters Volume 44, 2011 - Issue 8
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LIQUID CHROMATOGRAPHY

Quantitation of the Flavonols Quercetin and Kaempferol in the Leaves of Trigonella foenum-graecum by High-Performance Liquid Chromatography – Diode Array Detection

Evagelos Gikas GAIA Research Center, Bioanalytical Department, The Goulandris Natural History Museum, Kifissia, Greece
,
Fotini N. Bazoti GAIA Research Center, Bioanalytical Department, The Goulandris Natural History Museum, Kifissia, Greece
,
Nikolaos Papadopoulos GAIA Research Center, Bioanalytical Department, The Goulandris Natural History Museum, Kifissia, Greece
,
Alexandra Alesta Department of Crop Science, Agricultural University of Athens, Athens, Greece
,
Garyfalia Economou Department of Crop Science, Agricultural University of Athens, Athens, Greece
&
Anthony Tsarbopoulos GAIA Research Center, Bioanalytical Department, The Goulandris Natural History Museum, Kifissia, Greece; Department of Pharmacy, Laboratory of Pharmaceutical Analysis, University of Patras, Rio, GreeceCorrespondence[email protected]; [email protected]
Pages 1463-1472 | Received 29 Mar 2010, Accepted 02 Jun 2010, Published online: 14 Jun 2011
 
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Abstract

Trigonella foenum-graecum is known for the strong hypoglycemic effects of the seed extract and for its significant allelopathic effects. The leaf extract however has not been studied thoroughly up-to-date and no data are available regarding the quantitative content of the plant's leaves. The purpose of this study was to quantify the flavonoid content and to assess the flavonoid seasonal variation in the leaves of the plant during the three developmental stages, vegetative, flowering, and fruiting. An HPLC method has been developed and validated employing a reversed phase C18 column and Diode Array Detection. Sample pre-treatment was performed by methanol:water (80:20 v:v) extraction and glucoside hydrolysis with HCl. The method allowed the reliable determination of the aforementioned substances in the Trigonella's leaves revealing the highest amount of flavonoids during the flowering stage.

Notes

a Solvent A: HPLC-grade water H3PO4 1% (v/v).

b Solvent B: acetonitrile H3PO4 1% (v/v).

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