Abstract
Natural polymer polysaccharides chitosan (CS) was successfully assembled with phytic acid (PA) into {PA/CS}n layer-by-layer films. Myoglobin (Mb) could be gradually “absorbed” or loaded into {PA/CS}n films when the films were immersed into Mb solutions, forming {PA/CS}n-Mb films. The {PA/CS}n-Mb films demonstrated well-defined and quasi-reversible cyclic voltammetry (CV) responses for Mb FeIII/FeII redox couple and were used to catalyze electrochemical reduction of oxygen and hydrogen peroxide. The interaction between Mb and {PA/CS}n films was explored and discussed, which suggested that the electrostatic attraction might play a major role in loading Mb into the films. This new kind of film incorporated with redox proteins could be used to fabricate the new type of biosensors or bioreactors without using mediators.
Acknowledgments
The financial support from the National Natural Science Foundation of China (NSFC 20975015 and 21105004) and the Research Fund for the Doctoral Program of Higher Education of China (Project for Junior Faculty Members, 20070027021) is acknowledged.
Notes
This paper was submitted as part of a Special Issue on Biosensors organized by Dr. Yu Lei of the University of Connecticut.