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Analytical Letters Volume 45, 2012 - Issue 9
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BIOSENSORS

Label-Free Electrochemical Detection of Tetracycline by an Aptamer Nano-Biosensor

Juankun Zhang Tianjin Key Lab of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, ChinaCorrespondence[email protected]
,
Yan Wu Tianjin Key Lab of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China
,
Binbin Zhang Tianjin Key Lab of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China
,
Min Li Tianjin Key Lab of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China
,
Shiru Jia Tianjin Key Lab of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China
,
Shuhai Jiang Tianjin Key Lab of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China
,
Hao Zhou Tianjin Key Lab of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China
,
Yi Zhang Tianjin Key Lab of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China
,
Chaozheng Zhang Tianjin Key Lab of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China
&
Anthony P. F. Turner Biosensors & Bioelectronics Centre, IFM-Linköping University, Linköping, Sweden
 show all
Pages 986-992 | Received 01 Sep 2011, Accepted 17 Nov 2011, Published online: 11 Jun 2012
 
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Abstract

A novel aptamer nano-porous silicon (PS) biosensor was investigated for the rapid determination of tetracyclines. Electrochemical impedance spectroscopy (EIS) was used to analyze the behavior of the sensor. The specific binding of tetracycline to the aptamer biosensor led to a decrease in impedance. The corresponding impedance spectra (Nyquist plots) were obtained when serial concentrations of tetracycline were added into the system. An equivalent electrical circuit was used to fit the impedance data. The linear range of the sensor was 2.1–62.4 nM.

Acknowledgments

The authors would like to thank the Ministry of Science and Technology of the People's Republic of China (2009GJA10047), Tianjin Municipal Science and Technology Commission (09ZCZDSFO4200), and the Tianjin University of Science and Technology for support of this project.

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