Abstract
Ciclopirox olamine is an antifungal agent widely used in dermatological formulations for topical application. The methods described for its determination usually present time-consuming sample treatment or require major human participation. Here we present a rapid flow system for the determination of ciclopirox olamine, based on the use of sequential injection analysis with terbium-sensitized luminescence detection. The reaction between terbium and the analyte is carried out on-line, recording the analytical signal at 300/545 nm (λex/λem). A detection limit of 15 µg L−1 and a R.S.D. of 3% (n = 10) were obtained. It is worth mentioning that the method here presented is the only one that uses a flow system for the determination of ciclopirox olamine.
The flow system was applied to the analysis of different pharmaceutical preparations available in the Spanish Pharmacopoeia. A recovery study was performed, obtaining satisfactory results. The high solution throughput obtained, 50 samples per hour, makes the method suitable for its implementation in pharmaceutical laboratories for routine analysis.
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Acknowledgments
This article is part of a Special Issue Automated on Flow Injection Techniques organized by Dr. Paraskevas Tzanavaras of Aristotelian University of Thessaloniki, Greece.
The authors are grateful to the “Consejería de Innovación, Ciencia y Empresa (Junta de Andalucía),” Project P07-FQM-02673, for financial support.
Notes
a Analyte concentration, 200 µg L−1.
L.D.R. = Linear dynamic range; D.L. = Detection limit; Q.L. = Quantitation limit; MEKC˭Micellar electrokinetic capillary chromatography.
a Means of three determinations.
b 10 mg g−1.
c 80 mg g−1.
d 15 mg g−1.
e 10 mg g−1.