An Electrochemical Immunosensor for Fullerenol Detection Based on the Generated Antibody

Abstract

As the nanomaterial fullerenol has attracted much attention in various fields, there is a need to develop convenient and effective detection methods for its determination. In this paper, the polyclonal antibody against fullerenol was obtained by immunization of rabbits with the fullerenol protein conjugate. Based on this new antibody against fullerenol, an electrochemical immunosensor was constructed by employing the redox couple to sense the generated antibody and fullerenol interaction. The peak current decreased after the interaction of fullerenol with the immunosensor. Using this strategy, the concentration of fullerenol in the range of 1 × 10⁻⁵ to 1 mg mL⁻¹ was detected with a good linear relationship, and the lowest detection limit was about 3 × 10⁻⁶ mg mL⁻¹. This immunosensor was used to detect and quantify fullerenol.

Keywords:

• Electrochemical immunosensor
• Fullerenol
• Polyclonal antibody

Acknowledgments

The research was supported by the National Basic Research Program of China (973 Program) (2012CB932601, 2009CB930204), the National Natural Science Foundation of China (21271174, 20971124), and the China Postdoctoral Science Foundation of 20090460522.