Solid-Phase Room-Temperature Fluorescence Using a Nylon Membrane for the Determination of 2-Naphthalene Sulfonic Acid

Abstract

Using a nylon membrane as the supporting material, a simple, high sensitivity solid-phase surface fluorescence system has been assembled. Compared with other supporting materials, the nylon membrane is easily available and inexpensive. According to SEM images and the characteristics of other membranes to enrich the analyte, it can be concluded that the nylon membrane concentrates the analyte. Several parameters such as the photomultiplier tube voltage, slit widths, incident angle, pore size of the membrane, and analysis conditions (pH, filtration volume, flow rate) of this system were optimized. The 2-naphthalene sulfonic acid was chosen to be the analyte. Satisfactory analytical figures were obtained under the optimized experimental conditions which included a linear calibration range from 1.0 × 10⁻³ to 0.16 µg/mL; a relative standard deviation of 5.67% (n = 7) at a concentration of 0.02 µg/mL; and a limit of detection equal to 8.55 × 10⁻⁴ µg/mL.

Keywords:

• 2-Naphthalene sulfonic acid
• Nylon membrane
• Solid-phase fluorescence

Acknowledgments

The authors gratefully acknowledge the financial support for this project from National Science Foundation of China (grant No: 21205041) and a grant from Research Center of Analysis and Test of East China University of Science and Technology, China.