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IMMUNOASSAY

An Enzyme-Linked Immunosorbent Assay for Determination of Dicyclanil in Animal Tissue

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Pages 2870-2878 | Received 10 Apr 2013, Accepted 09 Jun 2013, Published online: 21 Nov 2013
 

Abstract

Dicyclanil is a toxic, pyrimidine-derived insect growth regulator used in veterinary medicine for the prevention of myiasis. A competitive indirect enzyme-linked immunosorbent assay was developed firstly for the determination of dicyclanil in animal tissue. The antigen was prepared using the glutaraldehyde method and polyclonal antibodies were obtained by immunizing rabbits with a dicyclanil-bovine serum albumin conjugate. The antibody obtained was specific for dicyclanil with an IC50 of 9 ng/mL. Recoveries from sheep tissue and liquid milk were in the range of 52.5–72.4%, with coefficients of variation between 4.7% and 11.9%. The results were further confirmed by high performance liquid chromatography–tandem mass spectrometry.

Acknowledgments

This research was supported in part by the Project for Academic Human Resources Development in Institutions of Higher Learning under the Jurisdiction of the Beijing Municipality (PHR), and the National Eleventh Five-Year Scientific and Technological Support Plan (2011BAD34B01).

Notes

a The MRL for fat has been recommended to be increased to 150 µg/kg (CVMP 2000).

a Molar cross-reactivity (CRmolar) was calculated using units of nmol/mL for the IC50 concentrations of the chemicals (Xu et al. Citation2009).

MR, Mean recovery; CV, coefficients of variation.

Xiaolin Hou and Shoujun Cao contributed equally to this work.

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