Determination of Nitidine Chloride in Toothpaste by Dual Gradient Two-Dimensional High-Performance Liquid Chromatography

Abstract

Nitidine chloride in toothpaste was determined by two-dimensional high-performance liquid chromatography. One gradient pump was used to enrich and purify the sample. The analyte was passed into the analytical column by switching a valve following the removal of the sample matrix. The samples were extracted by ultrasonic extraction. A C₁₈ (2.1 mm × 100 mm, 5 µm) column was used in one dimension, and a second C₁₈ (4.6 mm × 250 mm, 5 µm) column was used in the other dimension. The mobile phase was 0.02 mol/L monobasic potassium phosphate and acetonitrile with double isocratic elution. The wavelength of the ultraviolet-visible detector was 328 nm. The linear range for nitidine chloride was between 0.01 and 2 mg/L, with a correlation coefficient greater than 0.9998. The limit of detection for nitidine chloride was 0.01 mg/kg. The mean recoveries were 88.7% to 91.0%, and the relative standard deviation was less than 2.81%. This method is simple, sensitive, and accurate for the determination of nitidine chloride in toothpaste.

Keywords:

• Dual gradient system
• Nitidine chloride
• Toothpaste
• Two dimensional liquid chromatography