Determination of Arbutin, Niacinamide, and Adenosine in Functional Cosmetic Products by High-Performance Liquid Chromatography

Abstract

A reversed-phase high-performance liquid chromatography method using a diode array detector at 260 nm was developed and validated for the determination of arbutin, niacinamide, and adenosine in cosmetics. The analytes were extracted using methanol and deionized water. The separations were performed on a C₁₈ column with gradient elution. At the optimized conditions, the limits of detection for arbutin, niacinamide, and adenosine were 0.75, 0.12, and 0.01 µg/mL, respectively. The average recoveries were between 98.1% and 107.5%. The method was used to analyze thirty cosmetics.

Keywords:

• Adenosine
• Arbutin
• Functional cosmetics
• HPLC
• Niacinamide

Notes

^a y: peak area of analyte; x: concentration of analyte (µg/mL).

*Concentration of adenosine.

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