Abstract
A facile method based on liquid chromatography coupled with triple quadrupole mass spectrometry was established to determine floridoside and isofloridoside in red algae. Correlation coefficients of the calibration curves were larger than 0.9989, indicated good linearity. Detection limits of floridoside and isofloridoside were 0.05 and 0.20 ng/mL, respectively, and the limits of quantification were 0.1 and 0.4 ng/mL. The recoveries varied from 75.7% to 76.8%, and relative standard deviations of inter-day and intra-day precision were lower than 8.5% (n = 5). The effects of sea level variations in the intertidal zone on the osmotic role of floridoside and isofloridoside concentrations in seven red algae were investigated. It was shown that algae that inhabit higher levels in the intertidal zone contained higher concentrations of floridoside and isofloridoside. The results suggest that the presence of direct sun, exposure time, and temperature influenced to the concentrations of floridoside and isofloridoside due to the osmotic pressure adjustments.
Notes
a not detected.