Synthesis and Characterization of Silver Nanoparticle Modified 3-Aminophenol Resin Microspheres with Application for Determination of Carcinoembryonic Antigens by Surface-Enhanced Raman Scattering

Abstract

Uniform phenolic resin microspheres were prepared by the polycondensation of 3-aminophenol and formaldehyde. On the surface of the 3-aminophenol resin microspheres, silver nanoparticles were synthesized in situ and immobilized by simple heating. The composite was employed as a substrate for surface-enhanced Raman scattering (SERS). The SERS enhancement factor was evaluated using 4-mercaptobenzoic acid and Nile blue A as signal molecules. A highly sensitive SERS immunoassay that combined labeled antibody conjugated silver nanoparticle modified 3-aminophenol resin microspheres and coating antibody conjugated magnetic nanoparticles was fabricated to determine carcinoembryonic antigen. A linear relationship was obtained between the Raman intensity and the concentration of carcinoembryonic antigen. The limit of detection was 1.2 picograms per milliliter at a signal-to-noise ratio of three. This is believed to be the first report of a SERS immunoassay using silver nanoparticle modified 3-aminophenol resin microspheres as substrates.

Keywords::

• Carcinoembryonic antigen
• Immunoassay
• Phenolic resin microspheres
• Surface-enhanced Raman scattering