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ABSTRACT
In this work, the first use of a cellulose hydrogel film reconstituted from ionic liquid (IL) 1-butyl-3-methylimidazolium chloride for the fluorescent determination of a plant antimalarial endoperoxide artemisinin is reported. The sensing material was fabricated by noncovalent co-immobilization of the fluorescent cationic dye pyronin B and complex of Mn(II) with anionic surfactant sodium dodecyl sulfate into the film prepared by dissolution and regeneration of microcrystalline cellulose in ionic liquid. Artemisinin determination in a concentration range of 0.25–8 µM is based on the dynamic quenching of pyronin B fluorescence (emission wavelength/excitation wavelength of 581/355 nm) that is accelerated by the above-indicated complex. The developed disposable cellulose film exhibits a high sensitivity toward artemisinin (limit of detection of 30 nM), sufficient selectivity for pharmaceutical analysis, a rapid response time (30 s), and a strong stable fluorescent signal for over a month. The applicability of the cellulose film was demonstrated by analyzing a dietary supplement with an extract from the traditional Chinese herb Artemisia annua. The accuracy of the fluorescent determination of artemisinin in the dietary supplement was supported by a liquid chromatography–mass spectrometry technique. The developed fluorescent cellulose film is a biocompatible and easy to handle sensing material that makes it suitable for wide variety applications in pharmaceutical analysis.
Acknowledgments
The authors thank Research Scientist A. Stavrianidi (Department of Chemistry, Analytical Chemistry Division, Lomonosov Moscow State University) for artemisinin determination by the LC–MS method and Dr Prof. A. Terent’ev (N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences) for helpful discussions.