Isolation and Determination of Beta-Carotene in Carrots by Magnetic Chitosan Beta-Cyclodextrin Extraction and High-Performance Liquid Chromatography (HPLC)

Abstract

Beta-carotene is a carotenoid with strong antioxidant activity that has been used in many areas and attracted significant attention. Among the articles reported, however, there are few examples of the convenient and eco-friendly use of magnetic chitosan biopolymers to extract beta-carotene. This paper reports the ultrasonic-assisted solid phase extraction of beta-carotene. The beta-carotene in carrot samples was extracted using synthesized magnetic chitosan beta-cyclodextrin biopolymers prior to high-performance liquid chromatography (HPLC) detection. Under the optimal conditions, the beta-carotene content of carrot was found to be 41.06 ± 0.02 μg/g. This newly developed method using magnetic chitosan cyclodextrin biopolymers is a promising method for the ultrasonic-assisted solid phase extraction of carotenoids from vegetables because the procedure is facile and rapid and the magnetic biopolymers can be removed using simple process.

Keywords:

• Beta-carotene
• Fourier transform-infrared (FT-IR) spectroscopy
• high-performance liquid chromatography (HPLC)
• magnetic chitosan beta-cyclodextrin biopolymers
• thermogravimetric analysis (TGA)

Acknowledgments

This study was supported by an Inha University Research Grant (2019).

Disclosure statement

The authors declare that they have no financial and personal relationships with other individuals or organizations that inappropriately influenced this work.

Ethical statement

This article does not report any work using human participants or animals.