Simultaneous Analysis for the Multiple Periodontal Pathogens by High-Speed Capillary Electrophoresis

Abstract

In the present study, one important branch of high-speed capillary electrophoresis (HSCE), short-end CE, was employed for the highly efficient analysis of Porphyromonas gingivalis (Pg), Treponema denticola (Td), and Tannerella forsythia (Tf), which are closely related to the occurrence of periodontal disease. Aiming to realize rapid and simultaneous detection, a 100 bp DNA ladder marker ranging from 100 to 1500 bp was employed as the standard for the optimization of polymer concentration and electric field strength in developed protocol. The results showed that the PCR (polymerase chain reaction) products of Pg (197 bp), Td (311 bp), and Tf (641 bp) were simultaneously identified and quantitated in approximately 90 s using electrophoretic conditions that included 0.4% hydroxyethylcellulose (HEC, 1300 K) and a 200 V/cm electric field with an effective length of separation equal to 2.5 cm. The detected concentrations of the above target samples, determined by a time-modified internal standard route, were 1.08, 1.60, and 2.46 ng/µl, respectively. The present short-end CE strategy featured favorable properties for reproducibility, accuracy and linearity, whose evaluation included the values of relative standard deviation and the correlation coefficient. The current work may provide an effective avenue for the clinical detection of periodontal disease.

Keywords:

• High-speed capillary electrophoresis
• periodontal disease
• Porphyromonas gingivalis
• Tannerella forsythia
• Treponema denticola

Disclosure statement

The authors declare no conflicts of interest.

Additional information

Funding

The project was sponsored by Shanghai Sailing Program, China [No.18YF1422500], Research start-up project of Shanghai Institute of Technology [No.YJ2018-9].