206
Views
4
CrossRef citations to date
0
Altmetric
Biosensors

Electrochemical Biosensor Based on Polyelectrolyte Complexes with Dendrimer for the Determination of Reversible Inhibitors of Acetylcholinesterase

, , , & ORCID Icon
Pages 1709-1728 | Received 21 Jul 2020, Accepted 06 Sep 2020, Published online: 21 Sep 2020
 

Abstract

An acetylcholinesterase biosensor has been developed based upon a fourth generation poly(amidoamine) dendrimer with terminal amino groups. The immobilization of acetylcholinesterase was performed by physical adsorption onto the polyelectrolyte complex consisting of dendrimer and poly(styrene sulfonate) or deoxyribonucleic acid as counter ions. The efficiency of immobilization was evaluated by surface plasmon resonance that indicated a twofold increase of the quantity of enzyme on the surface in comparison with similar biosensors with no dendrimer. This property can be attributed to higher roughness of the underlying surface and its possible porosity caused by the star-shaped dendrimer molecules. The influence of the polyelectrolytes used on the biosensor performance as well as kinetic characteristics of enzymatic hydrolysis of thiocholine were assessed. The calibration curves of reversible inhibitors used in the therapy of neurodegenerative diseases (Huperzine A and galantamine) were ascribed with a four-parametrical logistic model. The 15% inhibition levels varied from 9.4 to 65 nM Huperzine A and 90 to 107 nM galantamine depending on the content of the polyelectrolyte complex. The biosensors were tested on example artificial and real urine samples and provided recoveries from 95 to 110%.

Additional information

Funding

This work was funded by the subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities (grant number 0671-2020-0063).

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 768.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.