Determination of Thiophanate-Methyl and Carbendazim from Environmental Water by Liquid-Liquid Microextraction (LLME) Using a Terpenoid-Based Hydrophobic Deep Eutectic Solvent and High-Performance Liquid Chromatography (HPLC)

Abstract

A hydrophobic deep eutectic solvent (DES) based vortex-assisted liquid-liquid microextraction (LLME) procedure was developed for the effective and rapid determination of thiophanate-methyl (TM) and carbendazim (CA) in lake water and tap water samples, with an excellence extraction efficiency (recovery: 87.2% to 97.4%, enrichment factor: 87.2 to 97.4). Twelve hydrophobic DESs based on terpenoids and fatty acids/alcohols were prepared, and those with the highest recovery for TM and CA were selected. The main parameters, including assisting methods, molar ratio of DES components, volume of DES, and NaCl content that may affect vortex-assisted LLME, were optimized. The intra-day and inter-day relative standard deviation (RSD) of this method were from 1.7% to 3.8% and 1.8% to 4.1%, showing excellent precision. The proposed method exhibited suitable limits of detection (TM: 0.053 µg/mL; CA: 0.007 µg/mL) and limits of quantification (TM: 0.177 µg/mL; CA: 0.024 µg/mL).

Keywords:

• Hydrophobic deep eutectic solvent
• liquid -liquid microextraction (LLME)
• high-performance liquid chromatography (HPLC)
• carbendazim
• thiophanate-methyl

Disclosure statement

The authors declare that they have no competing interests.

Additional information

Funding

This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT: Ministry of Science and ICT) (grant number NRF-2019R1A2C1010032).