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Analytical Letters Volume 26, 1993 - Issue 10
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Bioanalytical

Flow-Microcalorimetric Determination of Cellobiase Activity and Its Inhibition by Glucose

M. Beran Food Research Institute, Radiová 7, 102 31 Praha 10, Czechoslovakia
&
V. Paulíček Food Research Institute, Radiová 7, 102 31 Praha 10, Czechoslovakia
Pages 2107-2112 | Received 09 Feb 1993, Accepted 22 Apr 1993, Published online: 23 Sep 2006
 
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Abstract

A method for measuring cellobiase activity of the Trichoderma reesei CCF 1853 cellulase complex using a Thermal Activity Monitor and a flow - mix mode is described. The kinetic constant KM and the linear dependence of dQmax/dt (the maximum heat flow at the total saturation of enzyme with substrate) on the enzyme concentration were determined. The process of the end product inhibition of cellobiase activity by glucose has been observed too. The obtained results allow to determine the mechanism of the inhibition and an inhibition constant for glucose.

The procedure is completely general in nature and is applicable to other enzymatic systems.

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